2 Pharmaceutical Analysis IV
1 Bragg Equation Used for.....
• a) Analysis of crystal structure
• b) Analysis of Nature structure
• c) Analysis of Properties structure
• d) None of the above
2 In x-ray diffraction technique, used x-ray tube containing anode electrode which of the following.....
• a) Platinum
• b) Plutonium
• c) Tungsten
• d) Above a & c correct
3 Which of the following correct Bragg’s equation?
• a) N=2d (sin Q)
• b) ᶯλ= 2d (sin Q)
• c) ᶯλ= 2 (sin Q)
• d) None of the above
4 £ Max Vs λ Max longer wavelength shift is.....
• a) Hypsochromic shift
• b) Bathochromic shift
• c) Hypochromic shift
• d) Hyperchromic shift
5 In x-ray crystallography which type of nature of the compound analyse.
• a) Amorphous drug
• b) Powder drug
• c) Liquid drug
• d) All of the above
6 In which Chromatography s.p. is more polar than m.p. ?
• a) Ion exchange
• b) Liquid liquid Chromatography
• c) Reversed chromatography
• d) None of the above
7 Which force is involved in the Chromatography ?
• a) Hydrogen bonding
• b) London force
• c) Electric static force
• d) All of the above
8 Which technique is also known as colour writing ?
2 Pharmaceutical Analysis IV
• a) NMR
• b) Mass spectroscopy
• c) Chromatography
• d) IR spectroscopy
9 Chromatogram is?
• a) Solute concentration vs Elution time
• b) Solute concentration vs Elution volume
• c) A and B
• d) None of the above
10 Ion-exchange chromatography is used for the separation of
• a) Polar molecules
• b) Non polar molecules
• c) Semi polar molecules
• d) Highly polar molecule
11 Below the quantitative identification mark for solute in chromatography technique.
• a) Retention factor
• b) Retention time
• c) Capacity factor
• d) A and C correct
12 Affinity chromatography is used for the identification compound.
• a) Antibiotics/Enzyme
• b) Hydrophilic molecule
• c) Amino acid
• d) Proteins/ Polymer
13 Paper chromatography is used for the identification compound.
• a) Antibiotics/Enzyme
• b) Hydrophilic molecule
• c) Amino acid
• d) Proteins/ Polymer
14 Size-exclusion chromatography is used for the identification compound.
• a) Antibiotics/Enzyme
• b) Hydrophilic molecule
• c) Amino acid
• d) Proteins/ Polymer
15 Which of the following complete chromatography techniques.
• a) Liquid chromatography
• b) Gas chromatography
• c) Column chromatography
• d) Thin layer chromatography
2 Pharmaceutical Analysis IV
16 Retention Time calculated in Chromatography.....
• a) Cm/Min
• b) Cm/Pc
• c) Cm/Hr
• d) Cm/sec
17 Retention time and retention volume differentiated during development of chromatogram
• Temperature
• Pressure
• Flow rate
• a & c correct
18 Which polymer is often used for matrix materials in Affinity chromatography?
• a) Agarose
• b) Cellulose
• c) Dextrose
• d) a, b & c correct
19 Reversed-phase chromatography uses a stationary phase which is in nature.
• a) Hydrophilic
• b) Hydrophobic
• c) Both of above
• d) None of above
20 Which of the following techniques is used to study the three-dimensional structure of a molecule?
• a) Infra-red spectroscopy
• b) Mass spectrometry
• c) UV-visible spectroscopy
• d) X-ray crystallography
21 Infra-red spectroscopy exploits the change in what kind of behaviour in the molecules it is used to study?
• a) Molecular vibrations
• b) Nuclear spins
• c) Electron spins
• d) Electronic transitions
22 Which of the following ionizing detectors is used in gas chromatography.
• a) FID
• b) TCD
• c) ECD
• d) FPD
23 Flame ionizing detectors is more sensitive compound
2 Pharmaceutical Analysis IV
• a) Organophosphate Compound
• b) Nitrogenous compound
• c) a & b correct
• d) None of the above
24 Which of the following carrier gas used in GC.
• a) Helium
• b) Argon
• c) Hydrogen
• d) Oxygen
• e) a, c & d correct
25 Precolumn used in which type of chromatography.
• a) HPLC
• b) HPTLC
• c) GC-MS
• d) LC
26 Correct statement for guard column…
• a) it remove the impurities from the solvent
• b) it has a large particle size.
• c) short length of 2 to 10 cm, & not affect separation.
• d) prevents contamination of the analytical column
• e) All of the above correct
27 In HPLC Technique most commonly used detector is
• a) FPD
• b) PDA
• c) RI
• d) Conductivity
28 In HPTLC; if tiling effect produced_ added.
• a) acetic acid
• b) ammonia
• c) sulphuric acid
• d) a & b correct
29 TLC & HPTLC plate visualise on range.
• a) 260nm
• b) 254nm
• c) 255nm
• d) 280nm
30 Ion exchange resin have following properties_
• a) sufficient degree of cross linking 4 – 8%
• b) insoluble in solvents.
• c) chemically stable.
• d) Particle size must be as small as possible.
• e) all are correct
31 Which of the following statements regarding IR spectroscopy is wrong?
• a) Infrared radiation is higher in energy than UV radiation.
• b) Infrared spectra record the transmission of IR radiation.
2 Pharmaceutical Analysis IV
• c) Molecular vibrations are due to periodic motions of atoms in molecules, and include bond stretching, torsional changes, and bond angle changes.
• d) Infrared spectra give information about bonding features and functional groups in molecules.
32 Absorption of radiation in the UV range attributable to n→Ï€* electronic transitions is characteristic of which of the following types of compounds?
• a) Aromatic hydrocarbons.
• b) Unsaturated carbonyl compounds.
• c) Non-conjugated polyenes.
• d) Conjugated polyenes.
33 Which of the following statements is wrong.
• a) The wavenumber of a band in an IR spectrum is proportional to the frequency of the associated molecular vibration.
• b) Water is a good solvent for recording UV spectra of water-soluble compounds.
• c) Water is a good solvent for recording IR spectra of water-soluble compounds.
• d) Hydrogen bonding in hydroxyl compounds leads to broadening of spectral bands attributable to O-H stretching vibrations.
34 For calibration of IR Spectroscopy following_ used.
• a)Polypropyline
• b) Polystyrene
• C) Polypyrolidine
• d) Kbr
35 Mass no is Even & Atomic no is odd then spin quantum no......
• a) 1/2; 3/2 ; 5/2
• b) 0
• c) 1; 2; 3
• d) None of the above
36 NMR Spectroscopy not affect the energy except.
• a) Redio frequency
• b) Vibration
• c) Rotational
• d) Electronic
37 Example of shief reagent used for broadening of peak
• a) Europhium & Prasmidium
• b) Platinum & Europhium
• c) Vanadium
• d) None of the above
38 Which one of the correct graph of IR
• a)
2 Pharmaceutical Analysis IV
• c)
• d)
39 Fundamental region of ir
• a) 2 & 15 µm
• b) 0.8 to 15 µm
• c) 15 to 1000 µm
• d)100 to 1000 µm
40 By using cryogenic shims, field homogeneity can be as good as
• a. 1 ppm
• b. 10 ppm
• c. 1 ppb
• d. 0.01 ppm
41 The nuclear relaxation characterized by T1 relaxation is not
• a. spin–lattice relaxation
• b. longitudinal relaxation
• c. spin–spin relaxation
• d. relaxation along the z axis 42 Detector used in SLC based on
• a) chemical property
• b) Physical property
• c) Both a & b
• d) None of the above
43 The column in ion exchange chromatography is operated by:
• a) frontal Analysis
• b) Elution development
• c) Displacement development
• d) All of the above
2 Pharmaceutical Analysis IV
44 The commonly used cross linking agent is
• A) Divenylbenzene
• b) silica gel
• c) polystyrene
• d) celite
45 Reciprocating pumps offer a
• a) Non-pulsating flow
• b) Pulsating flow
• c) variable flow
• None of the above
46 Most commonly sp for HPLC contain:
• a) Particles of alumina
• b) Particles of silica
• c) Kiselguar
• d) None of the above
47 Commonly used locating agent for amino acid
• a) Ninhydrine agent
• b) Valanine reagent
• c) Chloroform
• d) CCl4
48 The approximate value of methyl proton in NMR is
• a) 1.5
• b) 0.5
• c) 0.9
• d) 1.2
49 Signal splitting in NMR arises from
• (A) Shielding effect
• (B) Spin-spin decoupling
• (C) Spin-spin coupling
• (D) Deshielding effect
50 In NMR spectrum the nuclei in up field resonate at
• (A) High frequency
• (B) Low frequency
• (C) It is constant throughout the spectrum
• (D) It doesn’t depends on chemical shift
51 The possible number of peaks in 1H NMR spectrum of the following structure is
•
• A) 1
• B) 2
• C) 3
• D) 4
52 The peak due to the proton attached to the first carbon in the above is splitted in to
• (A) Singlet
• (B) Doublet
• (C) Triplet
• (D) Quartet
2 Pharmaceutical Analysis IV
53 Chemical shift has the units of
• (A) Parts per million
• (B) Parts per billion
• (C) Tesla
• (D) No units
54 Coupling constant of two nuclei is defined as
• (A) Ratio of chemical shifts
• (B) Distance between splitted peaks in Hz
• (C) Difference of chemical shifts
• (D) Ratio of absorption frequencies 55 Boltzmann distribution is expressed by
• (A) nH / nL=e*-Ï’EI/KT
• (B) nL/nH=e*-Ï’E/KT
• (C) nH / nL=e* -Ï’E /BKT
• D) None of the above
56 A group producing deshielding effect causes
• (A) Increase in chemical shift
• (B) Decrease in chemical shift
• (C) Splitting of the peaks
• (D) Converging of peaks
57 In NMR spectroscopy, the spinning nuclei in strong magnetic field must be irradiated by which of the following?
• a) Perpendicular and stronger field
• b) Perpendicular and weaker field
• c) Parallel and stronger field
• d) Parallel and weaker field 58 Qualitative analysis of GLC based on
• a) time required for the peak to appear
• b) peak height
• c) Peak areas
• d) None of the above
59 Following types of sample handle to obtained NMR spectra
• a) liquid
• b) solid
• c) gas
• d) None
60 following is used as mulling agent
• a) Nujol
• b) CO2
• c) Salt Plates
• d) None
2 Pharmaceutical Analysis IV
Answers:
1 a 11 d 21 a 31 a 41 c 51 b
2 d 12 a 22 a 32 b 42 c 52 d
3 b 13 b 23 c 33 c 43 d 53 a
4 d 14 d 24 e 34 b 44 a 54 b
5 d 15 d 25 a 35 c 45 b 55 b
6 c 16 d 26 e 36 a 46 b 56 a
7 d 17 d 27 b 37 a 47 a 57 b
8 c 18 d 28 d 38 a 48 c 58 a
9 d 19 b 29 b 39 a 49 c 59 a
10 a 20 d 30 e 40 a 50 b 60 a