BP701T. INSTRUMENTAL METHODS OF ANALYSIS (Theory)
UNIT –III
Paper chromatography- Introduction, methodology, development techniques, advantages, disadvantages and applications
Handwritten Notes On Paper chromatography :
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Paper chromatography:
Chromatography is a method used to separate or identify compounds based on their physical properties. It is an elegant, scientific and systematic technique for analyzing complex mixtures. It also plays an important role in analyzing the properties of drug preparations and natural products. In this method, a column is used to separate compounds based on their properties by applying different pressures, temperatures or elutions. This method can be difficult to understand without practicing it yourself.
The process of separating compounds based on their physical properties using chromatography separates compounds based on their physical properties using chromatography. A column consists of a series of closely spaced partitions connected together by bridges. Compounds are separated based on their physical properties by placing them in the column and then applying pressure. The compound with the highest partition coefficient will move through the column at a faster rate and be at the top when eluted. This method can be used to separate compounds based on their chemical properties, such as volatility or solubility. It can also be used to analyze compounds in complex mixtures such as natural products or drug preparations.
Chromatography is a useful and simple technique for identifying and classifying natural products. For example, it can be used to identify alkaloids found in plants, including coca leaf, khat and tobacco. Chromatography can also be used to identify essential oils from plants- such as mint, pine and lavender- and fruit - such as figs and grapes - as well as spices - such as ginger and turmeric - found in food. Chromatography is also used to analyze environmental samples for the presence of chemicals produced by living organisms, such as plants or animals.
The type of column used to separate compounds by chromatography depends on the chemical properties wanted to separate the compounds. A Paper Column is an ideal choice for separating volatile substances based on their characteristics because it allows rapid movement of compounds through the column without causing damage. Other columns are made from glass, quartz- glass, metal or other materials- depending on the property wanted to separate the compounds. For example, a refrigerator is used to separate less volatile substances based on their coldness since it slows down the movement of heat within them.
Chromatography is an easy and effective way to analyze complex mixtures using a paper chromatography column to separate compounds based on their characteristics using chromatography columns to separate compound s based on their characteristics using chromatography columns based on their physical properties.
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