Pharmacological screening methods notes pdf and Handwritten unit I,Unit II,Unit III,Unit IV
Syllabus:
Unit –I 08 Hours
Laboratory Animals:
Study of CPCSEA and OECD guidelines for maintenance, breeding
and conduct of experiments on laboratory animals, Common lab
animals: Description and applications of different species and strains
of animals. Popular transgenic and mutant animals.
Techniques for collection of blood and common routes of drug
administration in laboratory animals, Techniques of blood collection
and euthanasia.
Unit –II 10 Hours
Preclinical screening models
a. Introduction: Dose selection, calculation and conversions,
preparation of drug solution/suspensions, grouping of animals and
importance of sham negative and positive control groups.
Rationale for selection of animal species and sex for the study. b. Study of screening animal models for
Diuretics, nootropics, anti-Parkinson’s,antiasthmatics,
Preclinical screening models: for CNS activity- analgesic,
antipyretic,anti-inflammatory, general anaesthetics, sedative and
hypnotics, antipsychotic, antidepressant, antiepileptic, antiparkinsonism, alzheimer’s disease
Unit –III
Preclinical screening models: for ANS activity, sympathomimetics,
sympatholytics, parasympathomimetics, parasympatholytics, skeletal
muscle relaxants, drugs acting on eye, local anaethetics
Unit –IV
Preclinical screening models: for CVS activity- antihypertensives,
diuretics, antiarrhythmic, antidyslepidemic, anti aggregatory, coagulants, and anticoagulants
Preclinical screening models for other important drugs like antiulcer,
antidiabetic, anticancer and antiasthmatics.
Research methodology and Bio-statistics
Selection of research topic, review of literature, research hypothesis
and study design
Pre-clinical data analysis and interpretation using Students ‘t’ test
and One-way ANOVA. Graphical representation of data
Recommended Books (latest edition):
1. Fundamentals of experimental Pharmacology-by M.N.Ghosh
2. Hand book of Experimental Pharmacology-S.K.Kulakarni
3. CPCSEA guidelines for laboratory animal facility. 4. Drug discovery and Evaluation by Vogel H.G. 5. Drug Screening Methods by Suresh Kumar Gupta and S. K. Gupta
6. Introduction to biostatistics and research methods by PSS Sundar Rao and J
Richard